Thermo Fisher Scientific MTDH Monoclonal Antibody (OTI1B6), TrueMAB
MTDH 단백질을 인식하는 마우스 단클론 항체로, Western blot 및 IHC(P) 분석에 적합합니다. 동결건조 형태로 제공되며 재구성 시 1 mg/mL 농도로 사용 가능합니다. 인간 시료에 반응하며, 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1B6 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 70–582 of human MTDH produced in SF9 cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
Metadherin (also known as MTDH or LYRIC) is a metastasis adhesion protein localized with tight junction proteins ZO-1 and occludin in polarized epithelial cells. It is recruited during tight junction maturation rather than serving as a structural component.
Metadherin is overexpressed in breast cancer tissues and xenografts compared to normal breast tissue. It binds to lung vasculature via a C-terminal extracellular domain segment, a mechanism implicated in breast cancer metastasis. Blocking this domain or silencing MTDH expression can inhibit metastatic progression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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