Thermo Fisher Scientific Phospho-c-Jun (Ser63) Recombinant Rabbit Monoclonal Antibody (SY0297)
Phospho-c-Jun (Ser63) 단백질을 검출하기 위한 고특이성 재조합 토끼 모노클로날 항체. Western blot, IHC, ICC/IF에 사용 가능. 높은 재현성과 민감도, 동물 유래 성분 없는 포뮬레이션. 인산화된 c-Jun의 신호전달 연구에 적합.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000–1:4,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:500
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SY0297 |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Ser63 of Human c-Jun (aa 31–80) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809412 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning the specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to larger rabbit immune repertoire
Target Information
c-Jun is a transcription factor that recognizes and binds to the enhancer heptamer motif 5’-TGA[CG]TCA-3’. When phosphorylated by HIPK3, it promotes NR5A1 activity, increasing steroidogenic gene expression upon cAMP signaling stimulation. It is also involved in KRAS-mediated transcriptional activation of USP28 in colorectal cancer cells.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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