Thermo Fisher Scientific Phospho-STAT1 (Tyr701) Monoclonal Antibody (KIKSI0803), eFluor 450, eBioscience
Phospho-STAT1 (Tyr701) 단백질을 인식하는 KIKSI0803 단클론 항체로, eFluor 450 형광체가 결합된 제품입니다. Flow Cytometry에 최적화되어 있으며, 인간 세포의 phosphorylated STAT1 검출에 사용됩니다. Violet laser(405 nm) 기반 분석에 적합합니다.
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Thermo Fisher Scientific Phospho-STAT1 (Tyr701) Monoclonal Antibody (KIKSI0803), eFluor 450, eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.125 µg)/test | View 2 publications |
Product Specifications
| Specification | Description |
|---|---|
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | KIKSI0803 |
| Conjugate | eFluor™ 450 |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2574118 |
Additional Formats
Product Specific Information
This KIKSI0803 monoclonal antibody recognizes STAT1 phosphorylated on tyrosine 701. STAT proteins are activated by cytokine receptor binding and phosphorylation by JAK family kinases, leading to nuclear translocation and DNA binding for gene regulation. Phosphorylation of STAT1 on Tyr701 is essential for dimer formation and transcriptional activity.
Specificity was confirmed by ELISA, flow cytometry, and western blot.
Reported applications include intracellular staining followed by flow cytometric analysis.
Tested for use at 5 µL (0.125 µg) per test, suitable for 10⁵–10⁸ cells/test.
Recommended Protocol:
Use Protocol C: Two-step (Fixation/Methanol).
Protocols A and B are not compatible.
Detailed procedures are available in BestProtocols® – Staining Intracellular Antigens for Flow Cytometry.
Fluorochrome Information:
eFluor™ 450 is an alternative to Pacific Blue™, excited by Violet laser (405 nm) and emits at 445 nm. Ensure instrument compatibility.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
STAT1 is a transcription factor activated by interferons (α, β, γ), EGF, PDGF, IL6, and other cytokines. It regulates genes involved in cell growth, apoptosis, immune response, and lipid metabolism. Located on chromosome 2, STAT1 activation involves phosphorylation by JAK kinases, leading to nuclear translocation and transcriptional regulation.
Among STAT family members, STAT1, STAT3, STAT5a, and STAT5b show broad activation profiles. Two alternatively spliced isoforms of STAT1 have been described.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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