Thermo Fisher Scientific ID2 Monoclonal Antibody (ILCID2), eFluor 450, eBioscience
Flow cytometry용 ID2 단일클론 항체로, 마우스 및 인간 ID2 단백질을 인식합니다. eFluor 450 형광체 결합으로 405/445 nm에서 검출 가능하며, Foxp3/Transcription Factor Staining Buffer 세트와 함께 사용 권장됩니다. 연구용으로만 사용 가능합니다.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific ID2 Monoclonal Antibody (ILCID2), eFluor 450, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 1.0 µg/test
- View 3 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Published Species | Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | ILCID2 |
| Immunogen | Purified recombinant fragment of human ID2 (amino acids 1–134) expressed in E. coli |
| Conjugate | eFluor™ 450 |
| Additional Formats | APC, PE, PE-Cyanine7, Request custom conjugation |
| Excitation / Emission Max | 405 / 445 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_2735052 |
Product Specific Information
Description:
This ILCID2 monoclonal antibody recognizes mouse and human ID2. It requires the Foxp3/Transcription Factor Staining Buffer Set and performs poorly with standard IC Fixation & Permeabilization Buffer Set.
Applications Reported:
- Flow cytometric analysis.
Applications Tested:
- Tested by flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00).
- Recommended use ≤1.0 µg per test (100 µL final volume).
- Cell number: 10⁵–10⁸ cells/test (empirical).
- Antibody titration recommended for optimal performance.
Fluorochrome Information:
- eFluor™ 450 is an alternative to Pacific Blue™.
- Emission: 446 nm, Excitation: 405 nm (Violet laser).
- Ensure instrument compatibility.
Target Information
The inhibitor of DNA binding 2 (ID2) protein promotes the development of various cell types, including natural killer cells, erythroid precursors, and CD8⁺/CD4⁺ T-cells. Lack of ID2 promotes B-cell development. ID2 associates with E proteins and other HLH family members, preventing DNA binding and transcription activation. Activation of ID2 is essential for natural killer cell lineage development.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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