Thermo Fisher Scientific PTPRC Monoclonal Antibody (OTI2E7), TrueMAB
인간 CD45 단백질을 인식하는 Mouse IgG1 단클론 항체로, WB, IHC, ICC, Flow Cytometry 등에 사용 가능. 동결건조 형태로 제공되며 재구성 후 약 1 mg/mL 농도. 면역세포 표면 단백질 연구 및 신호전달 분석에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:400–1:4,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2E7 |
| Immunogen | Full length human recombinant protein of human PTPRC produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) expressed in all nucleated hematopoietic cells, comprising about 10% of lymphocyte surface proteins.
It is absent in non-hematopoietic cells and tissues. CD45 plays a critical role in lymphocyte development and antigen signaling by regulating Src-family kinases.
Multiple isoforms exist due to alternative splicing, differing in extracellular domains but sharing identical transmembrane and cytoplasmic domains.
CD45RA, one isoform, shows restricted expression among lymphoid subtypes.
CD45 regulates T- and B-cell receptor signaling, modulates cytokine receptor activity via JAK kinases, and influences cell survival and apoptosis pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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