Thermo Fisher Scientific ZEB2 Monoclonal Antibody (OTI1E12), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:150

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).

Target Information

ZEB2, also known as Smad-interacting protein 1, is a nuclear DNA-binding transcriptional repressor that interacts with activated SMADs.
Similar to ZEB1, it inhibits E-cadherin transcription and induces epithelial-mesenchymal transition (EMT), which regulates cell migration during embryonic development and wound healing.
ZEB2 may also protect cells from DNA damage-induced apoptosis, contributing to tumor progression.
It has been observed in the cytoplasm of various cancer tissues, suggesting regulated localization in normal and tumor cells.
Mutations in ZEB2 are associated with Hirschsprung disease and Mowat-Wilson syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.