Thermo Fisher Scientific CD3e Monoclonal Antibody (145-2C11), Brilliant Violet 605, eBioscience

Thermo Fisher Scientific CD3e Monoclonal Antibody (145-2C11), Brilliant Violet 605

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 1.0 µg/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 496 / 563 / 615 / 661 / 737
• Brilliant Violet 421 / 480 / 650 / 711 / 786
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 510 / 610-70S / 660-120S
• NovaFluor Red 685 / 700 / 710
• NovaFluor Yellow 610 / 660 / 690 / 700 / 730
• PE / PE-Cyanine5 / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5
• Super Bright 436 / 600 / 780
• Request custom conjugation available

Product Specific Information

The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. The epsilon chain is essential for proper assembly, trafficking, and surface expression of the TCR complex. CD3 is expressed by thymocytes and all mature T cells. Binding of 145-2C11 to TCR initiates intracellular signaling leading to activation, proliferation, or apoptosis depending on conditions.

Applications Reported: Suitable for flow cytometric analysis.
Applications Tested: Validated on mouse splenocytes; use ≤1.0 µg/test (100 µL final volume). Optimize empirically.

Brilliant Violet™ 605 (BV605):

• Emission at 605 nm, excitation at 405 nm (violet laser).
• Protect from light; sensitive to photo-induced oxidation.
• Use compatible staining buffers such as Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer™ (00-4409-75) to minimize non-specific polymer interactions.
• Compatible fixation: IC Fixation Buffer (00-8222-49) or 1-step Fix/Lyse Solution (00-5333-54), up to 3 days at 4°C in dark.
• Not compatible with methanol-based fixation.

Laser Compatibility: Violet Laser (Excitation 407 nm / Emission 605 nm).
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

The CD3 complex transduces antigen-recognition signals into T cells and regulates TCR surface expression. It includes CD3 gamma, delta, epsilon, and zeta subunits, members of the immunoglobulin superfamily encoded on chromosome 11. CD3 plays roles in TCR-induced growth arrest, survival, and proliferation. Expressed on 68–82% of peripheral blood lymphocytes and 65–85% of thymocytes. Not expressed on B or NK cells. Defects in CD3 genes are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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