Thermo Fisher Scientific CD3 Monoclonal Antibody (OKT3), Brilliant Violet 605, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (0.5 µg)/test

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• Alexa Fluor 700
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 563 / 615 / 661 / 737 / 805
• Brilliant Violet 421
• eFluor 450 / 506 / 660
• FITC
• Functional Grade
• NovaFluor Blue 510 / 585
• NovaFluor Yellow 590 / 660 / 730
• PE / PE-Cyanine7 / PE-eFluor 610
• PerCP-Cyanine5.5 / PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Custom conjugation available

Product Specific Information

The OKT3 monoclonal antibody binds to an epitope on the epsilon-subunit of the human CD3 complex. It has potent immunosuppressive properties and has been used in treatment of organ transplant rejection. CD3 subunits (gamma, delta, epsilon) are essential for TCR complex assembly and expression. CD3 is expressed by thymocytes and all mature T cells.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and validated for flow cytometry using human peripheral blood cells at 5 µL/test (0.5 µg/test).
  • Recommended cell count: 10⁵–10⁸ cells/test
• Fluorochrome: Brilliant Violet™ 605 (BV605)
  • Excitation: 407 nm
  • Emission: 605 nm
  • Laser: Violet

Handling and Buffer Recommendations

When using multiple polymer dye-conjugated antibodies, use:

• Super Bright Complete Staining Buffer (Product # SB-4401-42)
• Brilliant Stain Buffer™ (Product # 00-4409-75)
  to minimize non-specific polymer interactions.

Light Sensitivity

This tandem dye is sensitive to photo-induced oxidation. Protect from light during storage and staining.

Fixation

Samples may be stored up to 3 days at 4°C in the dark using:

• IC Fixation Buffer (Product # 00-8222-49)
• 1-step Fix/Lyse Solution (Product # 00-5333-54)
  Brilliant Violet™ 605 is not compatible with methanol-based fixation.

Trademark

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

The CD3 complex transduces antigen-recognition signals into T cells and regulates TCR surface expression. CD3 subunits (gamma, delta, epsilon, zeta) are immunoglobulin superfamily members encoded on chromosome 11. CD3 is expressed on 68–82% of peripheral blood lymphocytes and 65–85% of thymocytes, but not on B or NK cells. Defects in CD3 genes are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures.

제품 이미지