Thermo Fisher Scientific HIF1A Polyclonal Antibody
HIF1A 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, ICC 등 다양한 응용에 적합합니다. 인간, 생쥐, 소 등 여러 종에 반응하며, 항원 친화 크로마토그래피로 정제된 고품질 항체입니다.
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- PA116627
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- pk
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:300 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:300 |
| Immunocytochemistry (ICC/IF) | 1:10–1:500 |
| Gel Shift (GS) | Assay-Dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Bovine, Human, Mouse, Pig, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Fusion protein containing amino acids 432–528 of human HIF-1α |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_568572 |
Product Specific Information
This antibody shows 93% sequence homology with bovine and 90% with mouse.
Suggested positive control: Cos-7 hypoxia-induced nuclear extract, COS-7 nuclear extracts (treated).
Target Information
HIF1-alpha (HIF1A) is a subunit of HIF1, a transcription factor found in mammalian cells under reduced oxygen tension. HIF-1 is a heterodimer consisting of alpha and beta subunits, both belonging to the basic-helix-loop-helix PAS family of transcription factors.
Under hypoxic conditions, HIF-1 activates transcription of over 40 genes involved in oxygen delivery and metabolic adaptation, including erythropoietin, glucose transporters, glycolytic enzymes, VEGF, and HILPDA.
HIF1-alpha regulates hypoxia-mediated apoptosis, cell proliferation, and tumor angiogenesis. It interacts with p53 and MDM2 under hypoxia, influencing ubiquitination and degradation. HIF1A also induces NOX4 to maintain ROS levels and promote proliferation after hypoxia.
In humans, HIF1A is located on chromosome 14q. Its C-terminal binds p300, forming complexes that induce hypoxia-responsive genes such as VEGF. Hypoxia plays a critical role in diseases including ischemia, cancer, pulmonary hypertension, congenital heart disease, and COPD.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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