Thermo Fisher Scientific KLHL12 Monoclonal Antibody (OTI5C12), TrueMAB
Human KLHL12 단백질(aa177-405)에 특이적인 Mouse IgG1 단클론 항체로 Western blot에 최적화됨. Lyophilized 형태, PBS buffer(8% trehalose) 포함, 보존제 무첨가. 연구용으로만 사용 가능.
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Applications
- Western Blot (WB)
Tested Dilution: 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5C12 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 177–405 of human KLHL12 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using carrier-free antibody, perform an additional desalting step using Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL (Product #89882).
Target Information
KLHL12 acts as a substrate-specific adapter for the CUL3-based ubiquitin-protein E3 ligase complex, negatively regulating the Wnt signaling pathway through targeted ubiquitination and proteolysis of DVL3.
Tissue specificity: Highly expressed in testis and at lower levels in the submandibular salivary gland.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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