Thermo Fisher Scientific CD197 (CCR7) Monoclonal Antibody (3D12), Brilliant Ultra Violet 737, eBioscience

Applications

• Flow Cytometry (Flow): 5 µL (1.0 µg)/test

Product Specifications

Additional Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 563 / 661
• Brilliant Violet 421 / 480 / 711 / 786
• FITC
• NovaFluor Red 685 / 700 / 755
• NovaFluor Yellow 700
• PE, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 600 / 645 / 780
• Request custom conjugation

Product Specific Information

The 3D12 monoclonal antibody reacts with human CCR7 (CD197), also known as EBI-1. CCR7 is a G-protein-coupled chemokine receptor functioning as a receptor for 6Ckine/SLC (CCL19, CCL21). It is expressed on T cells and dendritic cells and is involved in migration, chemotaxis, and maturation processes.

• Optimal staining: Incubate ≥45 minutes at 2–8°C
• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and tested on human peripheral blood cells at 5 µL (1.0 µg) per test (100 µL final volume)

Fluorochrome Information:
Brilliant Ultra Violet™ 737 (BUV737) emits at 732 nm; for use with UV (355 nm) laser-equipped cytometers. Protect from light.

Buffer Recommendations:
When using multiple polymer dye-conjugated antibodies, use Super Bright Complete Staining Buffer (SB-4401) or Brilliant Stain Buffer (00-4409-75) to reduce non-specific interactions.

Fixation Compatibility:
Samples can be stored up to 3 days at 4°C in the dark using IC Fixation Buffer (00-8222) or 1-step Fix/Lyse Solution (00-5333). Compatible with short-term methanol-based fixation (≤1 hour).

Excitation: 355 nm
Emission: 732 nm
Laser: Ultraviolet

Target Information

CCR7 belongs to the G protein-coupled receptor family and mediates effects of Epstein-Barr virus on B lymphocytes. It is expressed in blood, bone marrow, lymph nodes, and intestines, particularly in lymphoid tissues and activated B/T lymphocytes. CCR7 regulates memory T cell migration to inflamed tissues and dendritic cell maturation. Ligands include CCL19 (ECL) and CCL21.

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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