Thermo Fisher Scientific Rat IgG2b kappa Isotype Control (eB149/10H5), Brilliant Ultra Violet 737, eBioscience

Thermo Fisher Scientific Rat IgG2b kappa Isotype Control (eB149/10H5), Brilliant Ultra Violet 737

Applications

• Flow Cytometry (Flow): Assay-Dependent
• Control (Ctrl): Assay-Dependent

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 561, 647, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395, 496, 563, 615, 661, 805
• Brilliant Violet 421, 480, 605, 650, 711, 786
• eFluor 450, 506, 570, 660
• FITC
• Functional Grade
• PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436, 600, 645, 702, 780
• Request custom conjugation available

Product Specific Information

The rat IgG2b kappa monoclonal antibody serves as an isotype control immunoglobulin.

• Applications Reported: Suitable for flow cytometric analysis.
• Applications Tested: Tested on mouse splenocytes and human peripheral blood cells; use at same concentration as experimental antibody.
• Fluorochrome: Brilliant Ultra Violet™ 737 (BUV737), emits at 732 nm, for UV (355 nm) laser-equipped cytometers.
• Buffer Recommendation: Use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409) to minimize non-specific polymer interactions.
• Light Sensitivity: Protect from light to prevent photo-induced oxidation.
• Fixation: Compatible with IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in the dark.
• Methanol Compatibility: Short-term methanol fixation possible; avoid long-term storage in methanol-containing buffers.
• Laser Specifications: Excitation 355 nm; Emission 732 nm; Ultraviolet Laser.

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen™.

Target Information

Rat IgG2b encodes the immunoglobulin G2b subclass of rat antibodies, important in immune response via pathogen neutralization and opsonization. Commonly used in immunohistochemistry, flow cytometry, and Western blotting.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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