Thermo Fisher Scientific ZIPK Polyclonal Antibody
ZIPK 단백질을 인식하는 Rabbit Polyclonal 항체로, Human, Mouse, Rat 시료에 반응합니다. Western blot 및 Immunocytochemistry에 적합하며, 항원 친화 크로마토그래피로 정제되었습니다. 세포 이동성, 수축, 세포사멸 연구에 활용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A peptide corresponding to amino acids near the center of human ZIP kinase. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long term storage store at -20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11153624 |
Product Specific Information
In Western blot applications, this antibody detects a band at approximately 40 kDa, representing the pro-enzyme of DNase II.
A suggested positive control is human spleen tissue lysate.
PA5-19902 can be used with blocking peptide PEP-0028.
Target Information
DAPK3 (ZIPK) is a serine/threonine kinase involved in cell motility, smooth muscle contractility, and cell death processes.
It is a component of desmosomes and the epidermal cornified envelope in keratinocytes.
The N-terminal domain interacts with the plasma membrane, while the C-terminus associates with intermediate filaments.
Through its rod domain, this protein forms complexes with envoplakin, linking the cornified envelope, desmosomes, and intermediate filaments.
AKT1/PKB, a kinase involved in cell growth and survival signaling, interacts with DAPK3, suggesting a role in AKT1-mediated signaling localization.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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