Thermo Fisher Scientific DNase II Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5 µg/mL

-

Immunohistochemistry (IHC)

5 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A peptide near the carboxy terminus of human DNase II. The immunogen is located within the last 50 amino acids of DNase II. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:DNase II,uniProtId:O00115-1,ncbiNodeId:9606,antigenRange:360,antigenLength:360,antigenImageFileName:PA5-19900_DNase_II_O00115-1_Rabbit.svg,antigenImageFileNamePDP:PA5-19900_DNase_II_O00115-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11154778

Product Specific Information

In Western blot applications, this antibody detects a band at ~40kDa, which represents the pro-enzyme of DNase II. A suggested positive control is human spleen tissue lysate.

PA5-19900 can be used with blocking peptide PEP-0026.

Target Information

DNase II is a member of the DNase family. The protein, located in the lysosome, hydrolyzes DNA under acidic conditions and mediates the breakdown of DNA during erythropoiesis and apoptosis. Two codominant alleles have been characterized, DNASE2*L (low activity) and DNASE2*H (high activity), that differ at one nucleotide in the promoter region. The DNASE2*H allele is represented in this record.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.