Thermo Fisher Scientific TCR V alpha 2 Monoclonal Antibody (B20.1), PE, eBioscience

Thermo Fisher Scientific TCR V alpha 2 Monoclonal Antibody (B20.1), PE, eBioscience

Applications and Tested Dilution

• Flow Cytometry (Flow): 0.5 µg/test
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Product Specifications

Available Formats

• APC
• APC-eFluor 780
• Biotin
• eFluor 450
• FITC
• NovaFluor Blue 610-70S
• NovaFluor Red 700
• NovaFluor Yellow 570 / 610 / 660
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436 / 600 / 780
• Request custom conjugation

Product Specific Information

The B20.1 monoclonal antibody reacts with mouse T-cell receptor (TCR) V alpha 2 chains and recognizes most TCR V alpha 2 subfamily members in mice with a, b, and c haplotypes. It also cross-reacts with TCR V delta 8 due to sequence homology.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Tested on mouse lymph node cells
• Recommended Usage: ≤ 0.5 µg per test (100 µL final volume, 10⁵–10⁸ cells/test)
• Preblocking: Fc receptors with 0.5 µg purified anti-mouse CD16/32 (Product #14-0161-85)
• Excitation: 488–561 nm
• Emission: 578 nm
• Laser: Blue, Green, Yellow-Green
• Filtration: 0.2 µm post-manufacturing filtered

Target Information

T cell receptors (TCR) recognize peptides presented by MHC class II molecules to mediate adaptive immune responses.

• Recognition of self-peptides is linked to autoimmune diseases.
• Mutant self-peptides are associated with tumors.
• Superantigen engagement of TCRs causes toxic shock syndrome.
• Autoantibodies to TCR V beta segments have been found in RA and SLE patients, potentially regulating TH1-mediated inflammation.
• Most human T cells express TCR αβ with CD4 or CD8, while a small subset (DN T cells) lacks both markers and may increase in autoimmune or immunodeficiency conditions.
• γδ T cells, found in epithelia, exhibit distinct antigen recognition and may have antitumor and immunoregulatory roles.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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