Thermo Fisher Scientific HIF1A Polyclonal Antibody
다양한 종에서 반응하는 HIF1A 폴리클로날 항체로, WB, IHC, ICC, ELISA, IP, ChIP 등 다양한 응용에 적합합니다. 항원 친화 크로마토그래피로 정제된 고품질 항체이며, 저산소 반응 및 세포 내 HIF1A 연구에 활용됩니다.
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Thermo Fisher Scientific HIF1A Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:500 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | Assay-dependent |
| Immunoprecipitation (IP) | 1:1,000 |
| ChIP assay (ChIP) | 2.5 µg/10^6 cells |
| ChIP-sequencing (ChIP-Seq) | Assay-dependent |
| Gel Shift (GS) | 1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Guinea pig, Human, Mouse, Non-human primate, Rat, Xenopus, Zebrafish |
| Published Species | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Fusion protein containing amino acids 432–528 of human HIF-1α |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2248222 |
Product Specific Information
- For ChIP, nuclear extracts are recommended.
- Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
- Suggested positive control: Cos-7 Hypoxia Induced nuclear extract Kit6.
Target Information
HIF1-alpha (HIF1A) is a subunit of HIF1, a transcription factor found in mammalian cells under reduced oxygen tension. HIF-1 is a heterodimer consisting of alpha and beta subunits, both belonging to the basic-helix-loop-helix PAS family of transcription factors. It regulates transcriptional responses to hypoxia, activating over 40 genes involved in oxygen delivery and metabolic adaptation.
HIF1-alpha is implicated in hypoxia-mediated apoptosis, cell proliferation, and tumor angiogenesis. Under hypoxic conditions, p53 interacts with HIF1-alpha, promoting MDM2-mediated ubiquitination and degradation. Induction of NOX4 by HIF1-alpha helps maintain ROS levels after hypoxia. In humans, HIF1A is located on chromosome 14q, and its C-terminal binds p300, forming complexes that induce hypoxia-responsive genes such as VEGF.
Hypoxia plays a major role in diseases including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease, and chronic obstructive pulmonary disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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