Thermo Fisher Scientific MRE11 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:5,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:500

-

Immunohistochemistry (Frozen) (IHC (F))

1:10-1:500

-

Immunocytochemistry (ICC/IF)

1:200

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-Dependent

-

ELISA (ELISA)

Assay-Dependent

-

Immunoprecipitation (IP)

3 µL

-

ChIP assay (ChIP)

Assay-Dependent

-

in situ PLA (PLA)

Assay-Dependent

-

Product Specifications

Species Reactivity

Chicken, Hamster, Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2145113

Product Specific Information

This Mre11 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections (PMID 21279473) Immunoprecipitation and Western Blot.

In WB, a band can be seen at ~81 kDa.

For ICC/IF, this antibody has been used with methanol-fixed IMR90 primary human fibroblasts. For IP, the suggested working dilution is 3 µL for immunoprecipitation of 3X10^6 cells.

Co-IP application has been reported by Ching et al 2012 (PMID: 22190719).

Suggested positive control: antigen standard for MRE11A (transient overexpression lysate), Hela whole cell extract, Hela whole cell lysate.

Target Information

MRE11 (double-strand break repair protein MRE11) is a component of the MRN complex. This complex plays a central role in double-strand break repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-stranded endonuclease activity and double-strand-specific 3-5 exonuclease activity which are provided by MRE11. RAD50 is required to bind DNA ends and hold them in close proximity. Mutations in the gene could result in ataxia-telangiectasia-like disorder 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.