Thermo Fisher Scientific Androgen Receptor Polyclonal Antibody

Thermo Fisher Scientific Androgen Receptor Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

PA1-111B detects androgen receptor (AR) from human samples.
This antibody does not detect estrogen, progesterone, or glucocorticoid receptors.

PA1-111B has been successfully used in Western blot procedures.
By Western blot, this antibody detects an ~110 kDa protein representing AR from HepG2 nuclear extracts.

The PA1-111B immunogen is a synthetic peptide corresponding to residues M(1) E V Q L G L G R V Y P R P P S K T Y R G(21) C of human AR.
This sequence is completely conserved between human, mouse, and rat AR.

The PA1-111B immunizing peptide (Cat. # PEP-006) is available for use in neutralization and control experiments.

Target Information

The androgen receptor (AR) is a member of the steroid-hormone receptor superfamily of nuclear receptors.
It has three major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain.
AR is a ligand-activated transcription factor that binds active testosterone (T) and dihydrotestosterone (DHT).

Upon hormone binding, AR dissociates from accessory proteins, translocates into the nucleus, dimerizes, and stimulates transcription of androgen-responsive genes.
AR signaling plays a key role in development and function of male reproductive organs, including the prostate and epididymis, and also in nonreproductive organs such as muscle, hair follicles, and brain.

Androgen Receptor is a phosphoprotein that regulates mitogen-activated protein kinase (MAP kinase).
Inhibition of the MEK1/2 pathway correlates directly with a change in phosphorylation state of AR.

Abnormalities in AR signaling have been linked to diseases including prostate cancer, Kennedy’s disease, and male infertility.
Mutations in this gene are associated with complete androgen insensitivity (CAIS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.