Thermo Fisher Scientific Androgen Receptor Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

PA1-110 detects androgen receptor (AR) from human, primate, rat, and mouse tissues.
This antibody does not detect estrogen, progesterone, or glucocorticoid receptors.

PA1-110 has been successfully used in Western blot, immunocytochemistry, immunohistochemistry, and immunoprecipitation procedures.
By Western blot, this antibody detects an ~110 kDa protein representing AR from rat brain homogenate.
Immunohistochemical staining of AR in rat brain with PA1-110 results in intense nuclear staining.

For fixation of frozen sections, 2% paraformaldehyde + 15% picric acid solution in phosphate buffer (pH 7.4) is recommended.
The PA1-110 immunogen is a fusion protein containing residues 331–572 of human androgen receptor.

Target Information

The androgen receptor (AR) is a member of the steroid-hormone receptor superfamily of nuclear receptors.
It is a ligand-activated transcription factor that binds active testosterone (T) and dihydrotestosterone (DHT).
Upon ligand binding, the receptor translocates into the nucleus, dimerizes, and activates transcription of androgen-responsive genes.

AR signaling is essential for male reproductive organ development and function (prostate, epididymis) and also affects nonreproductive tissues (muscle, hair follicles, brain).
AR is a phosphoprotein regulating MAP kinase activity.
Abnormal AR signaling is associated with diseases such as prostate cancer, Kennedy’s disease, and male infertility.
Mutations in this gene can cause complete androgen insensitivity (CAIS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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