Thermo Fisher Scientific PKM2 Monoclonal Antibody (OTI11G10), TrueMAB
PKM2 단백질을 인식하는 Mouse IgG1 단일클론 항체로 Western blot 및 IHC(P) 적용 가능. 합성 펩타이드 기반 면역원 사용, 고순도 친화성 크로마토그래피 정제. 동결건조 형태로 제공되며, -20°C 보관 권장.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI11G10 |
| Immunogen | Synthetic peptide sequence corresponding to the region encoded by PKM gene M2 isoform |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Synthetic peptide sequence: LRRLAPITSDPTEATAVGAV
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
PKM2 (Pyruvate Kinase M2) catalyzes the conversion of phosphoenolpyruvate to pyruvate and ATP.
It interacts with thyroid hormone, mediating metabolic effects, and binds Opa protein, a bacterial outer membrane protein involved in gonococcal adherence and invasion of human cells.
Three alternatively spliced transcript variants encoding two distinct isoforms of PKM2 have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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