Thermo Fisher Scientific Methylglyoxal Monoclonal Antibody (9E7), PerCP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:25-1:50

-

Immunocytochemistry (ICC/IF)

1:50

-

Flow Cytometry (Flow)

1:100

-

ELISA (ELISA)

1:1,000

-

Product Specifications

Species Reactivity

Chemical

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

9E7

Immunogen

Synthetic Methylglyoxal modified Keyhole Limpet Hemocyanin (KLH).

Conjugate

PerCP PerCP PerCP

View additional formats

• Unconjugated
• APC
• FITC
• PE
• Request custom conjugation

Excitation/Emission Max

482/675 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2932265

Product Specific Information

A 1:1,000 dilution of MA5-45811 was sufficient for detection of Methylglyoxal in 0.5 µg of Methylglyoxal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.|Specific for Methylglyoxal modified proteins. Does not detect free Methylglyoxal. Does not cross-react with Acrolein, Hexanoyl Lysine, Malondialdeyhde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Crotonaldehyde modified proteins.

Target Information

Methylglyoxal, also called pyruvaldehyde or 2-oxopropanal, is the organic compound with the formula CH3C(O)CHO. Gaseous methylglyoxal has two carbonyl groups, an aldehyde and a ketone but in the presence of water, it exists as hydrates and oligomers. Methylglyoxal is a reduced derivative of pyruvic acid.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.