Thermo Fisher Scientific Methylglyoxal Monoclonal Antibody (9E7), PE
Methylglyoxal 변형 단백질을 특이적으로 검출하는 단클론 항체. PE 형광 표지로 Flow Cytometry, WB, IHC, ICC, ELISA 등에 사용 가능. 마우스 IgG2a 형, 고순도 Protein G 정제. 보존제 없음, 4°C 보관.
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Thermo Fisher Scientific Methylglyoxal Monoclonal Antibody (9E7), PE
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:25–1:50 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| Flow Cytometry (Flow) | 1:100 |
| ELISA | 1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 9E7 |
| Immunogen | Synthetic Methylglyoxal modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932266 |
Available Formats
- Unconjugated (MA5-27567)
- APC (MA5-45809)
- FITC (MA5-45810)
- PerCP (MA5-45811)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45812 was sufficient for detection of Methylglyoxal in 0.5 µg of Methylglyoxal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for Methylglyoxal modified proteins. Does not detect free Methylglyoxal. Does not cross-react with Acrolein, Hexanoyl Lysine, Malondialdehyde, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Crotonaldehyde modified proteins.
Target Information
Methylglyoxal (pyruvaldehyde, 2-oxopropanal) is an organic compound with the formula CH₃C(O)CHO. It possesses both aldehyde and ketone groups. In aqueous environments, it exists as hydrates and oligomers and is a reduced derivative of pyruvic acid.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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