Thermo Fisher Scientific Malondialdehyde Monoclonal Antibody (11E3), PE
Malondialdehyde 단백질에 특이적인 단클론 항체로, PE 형광표지되어 다양한 면역분석(WB, IHC, ICC/IF, ELISA)에 사용 가능. 높은 특이성과 재현성을 제공하며, 단백질 G로 정제된 액상 형태로 제공. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:100 |
| Immunocytochemistry (ICC/IF) | 1:50 |
| ELISA | 1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 11E3 |
| Immunogen | Synthetic Malondialdehyde modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | PE (R-Phycoerythrin) |
| Excitation / Emission Max | 565 / 576 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932262 |
Available Formats
- Unconjugated (MA5-27560)
- APC (MA5-45805)
- FITC (MA5-45806)
- PerCP (MA5-45807)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45808 was sufficient for detection of Malondialdehyde in 2 µg of Malondialdehyde conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for Malondialdehyde conjugated proteins. Does not detect free Malondialdehyde. Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Methylglyoxal modified proteins.
Target Information
Malondialdehyde (CH₂(CHO)₂) is an organic compound and a byproduct of lipid metabolism. It is a highly reactive electrophilic species that forms covalent adducts with proteins, known as advanced lipoxidation end products (ALE).
Malondialdehyde also reacts with DNA bases such as deoxyadenosine and deoxyguanosine, forming mutagenic adducts. It is naturally present in various foods and found in high concentrations in rancid products.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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