Thermo Fisher Scientific JARID1B Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:10,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:2,000

-

Immunoprecipitation (IP)

2-10 µg/mg lysate

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Region between residue 1494 and 1544 of human Jumonji, AT rich interactive domain 1B. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:KDM5B,uniProtId:Q9UGL1-1,ncbiNodeId:9606,antigenRange:1494-1544,antigenLength:1544,antigenImageFileName:A301-813A_KDM5B_Q9UGL1-1_Rabbit.svg,antigenImageFileNamePDP:A301-813A_KDM5B_Q9UGL1-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

tris citrate/phosphate, pH 7-8

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Wet ice

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Target Information

KDM5B is a histone demethylase that demethylates Lys-4 of histone H3, thereby playing a central role in histone code. It does not demethylate histone H3 Lys-9 or H3 Lys-27. KDM5B demethylates trimethylated, dimethylated and monomethylated H3 Lys-4. It acts as a transcriptional corepressor for FOXG1B and PAX9. It favors the proliferation of breast cancer cells by repressing tumor suppressor genes such as BRCA1 and HOXA5. In contrast, KDM5B may act as a tumor suppressor for melanoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.