Thermo Fisher Scientific CDK2 Polyclonal Antibody
Rabbit polyclonal antibody targeting human CDK2 for WB, IHC, and IP applications. Recognizes residues 248–298 of CDK2, supplied as 1 mg/mL liquid form. Predicted to react with rat, bovine, and hamster; ideal for cell cycle and kinase research.
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Thermo Fisher Scientific CDK2 Polyclonal Antibody
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 248 and 298 of human cyclin-dependent kinase 2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections
- Based on 100% sequence identity, this antibody is predicted to react with Rat, Bovine, and Golden hamster
Target Information
CDK2/cyclinO is a member of the cyclin-dependent protein kinase family complexed with CyclinO. CyclinO is a specific protein isoform of the UNG gene, localized exclusively in the nucleus and exhibiting uracil-DNA glycosylase activity. CDK2/cyclinO protein levels increase during the G1 phase and are turned over during the cell cycle. The cyclin A–CDK2 complex peaks at the G1/S transition and associates with DNA in the initiation complex during replication. Rb protein acts as a substrate for CDK2–Cyclin E and/or CDK2–Cyclin A in vivo. CDK2 is activated and localized to the nucleus during late G1, S, and G2 phases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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