Thermo Fisher Scientific H3K27me3S28ph Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250

-

Immunocytochemistry (ICC/IF)

1:200

-

ELISA (ELISA)

1:100-1:500

-

Immunoprecipitation (IP)

5 µL/IP

-

ChIP assay (ChIP)

1 µL/ChIP

View 1 publication 1 publication

Dot blot (DB)

1:20,000

-

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Raised against histone H3 containing the trimethylated lysine 27 and the phosphorylated serine 28 (H3K27me3S28p), using a KLH conjugated synthetic peptide. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:H3K27me3S28ph,uniProtId:Q71DI3-1,ncbiNodeId:9606,antigenRange:27,antigenLength:136,antigenImageFileName:49-1015_H3K27me3S28ph_Q71DI3-1_Rabbit.svg,antigenImageFileNamePDP:49-1015_H3K27me3S28ph_Q71DI3-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

<0.1% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

RRID

AB_2533865

Target Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the telomeric copy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.