Thermo Fisher Scientific H3K27me3S28ph Polyclonal Antibody
H3K27me3S28ph 변형 히스톤에 특이적인 Rabbit Polyclonal 항체로, Western blot, ICC/IF, ELISA, IP, ChIP 등 다양한 응용에 사용 가능. Human 시료에 반응하며, -20°C에서 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:250 |
| Immunocytochemistry (ICC/IF) | 1:200 |
| ELISA | 1:100–1:500 |
| Immunoprecipitation (IP) | 5 µL/IP |
| ChIP assay (ChIP) | 1 µL/ChIP |
| Dot blot (DB) | 1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Raised against histone H3 containing the trimethylated lysine 27 and the phosphorylated serine 28 (H3K27me3S28p), using a KLH-conjugated synthetic peptide. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | <0.1% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Dry ice |
| RRID | AB_2533865 |
Target Information
Histones are basic nuclear proteins responsible for the nucleosome structure of chromosomal fibers in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). Chromatin fibers are further compacted through the interaction of a linker histone (H1) with DNA between nucleosomes, forming higher-order chromatin structures.
This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails and instead contain a palindromic termination element. The gene is located in a histone cluster on chromosome 1 and represents the telomeric copy among four duplicated histone genes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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