Thermo Fisher Scientific H3K4me2 Polyclonal Antibody
H3K4me2 변형 히스톤 H3를 인식하는 Rabbit Polyclonal 항체로, Western blot, ELISA, Dot blot 및 ChIP assay에 사용 가능. 인간 및 마우스 시료에 반응하며, 연구용으로 염색질 구조 및 전사 조절 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:750 | – |
| ELISA | 1:300 | – |
| ChIP assay (ChIP) | – | View 3 publications |
| Dot blot (DB) | 1:20,000 | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Raised against histone H3 containing the dimethylated lysine 4 (H3K4me2), using a KLH-conjugated synthetic peptide. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not Determined |
| Storage Buffer | Whole serum |
| Contains | <0.1% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Dry ice |
| RRID | AB_2533855 |
Target Information
Histone H3 is one of the DNA-binding proteins found in the chromatin of all eukaryotic cells. H3, along with four core histone proteins, binds to DNA forming the structure of the nucleosome. Histones play a central role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. Post-translationally, histones are modified in various ways to either directly change the chromatin structure or allow the binding of specific transcription factors.
The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of post-translational modification that influence cellular processes. These include methylation or acetylation of lysine and arginine residues and phosphorylation of serine or threonine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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