Thermo Fisher Scientific Angiotensinogen Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

-

Immunocytochemistry (ICC/IF)

1:50-1:100

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 35-485 of human Angiotensinogen (NP_0000201) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Angiotensinogen,uniProtId:P01019-1,ncbiNodeId:9606,antigenRange:35-485,antigenLength:485,antigenImageFileName:PA5-90591_Angiotensinogen_P01019-1_Rabbit.svg,antigenImageFileNamePDP:PA5-90591_Angiotensinogen_P01019-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.10 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2806132

Product Specific Information

Positive Samples: HepG2

Immunogen sequence: RVYIHPFHLV IHNESTCEQL AKANAGKPKD PTFIPAPIQA KTSPVDEKAL QDQLVLVAAK LDTEDKLRAA MVGMLANFLG FRIYGMHSEL WGVVHGATVL SPTAVFGTLA SLYLGALDHT ADRLQAILGV PWKDKNCTSR LDAHKVLSAL QAVQGLLVAQ GRADSQAQLL LSTVVGVFTA PGLHLKQPFV QGLALYTPVV LPRSLDFTEL DVAAEKIDRF MQAVTGWKTG CSLMGASVDS TLAFNTYVHF QGKMKGFSLL AEPQEFWVDN STSVSVPMLS GMGTFQHWSD IQDNFSVTQV PFTESACLLL IQPHYASDLD KVEGLTFQQN SLNWMKKLSP RTIHLTMPQL VLQGSYDLQD LLAQAELPAI LHTELNLQKL SNDRIRVGEV LNSIFFELEA DEREPTESTQ QLNKPEVLEV TLNRPFLFAV YDQSATALHF LGRVANPLST A

Target Information

The protein encoded by AGT, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.