Thermo Fisher Scientific CXCL6 Polyclonal Antibody, Biotin, PeproTech

Applications

Western Blot (WB)

• Recommended dilution: 0.1–0.2 µg/mL

ELISA

• Recommended dilution: 0.25–1.0 µg/mL

Product Specifications

Product Specific Information

Amino Acid Sequence of recombinant protein:
VLTELRCTCL RVTLRVNPKT IGKLQVFPAG PQCSKVEVVA SLKNGKQVCL DPEAPFLKKV IQKILDSGNK KN

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human GCP-2 (CXCL6).
Anti-Human GCP-2 (CXCL6)-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect Human GCP-2 (CXCL6) by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL is required.
This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human GCP-2 (CXCL6) (500-P120) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant Human GCP-2 (CXCL6).

Western Blot:
To detect hGCP-2 by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL.
Used with compatible secondary reagents, the detection limit for Recombinant hGCP-2 is 1.5–3.0 ng/lane under either reducing or non-reducing conditions.

Packaging:
500-P120BT-1MG will be provided as 2 × 500 µg.

Target Information

CXCL6 is a chemotactic factor for neutrophil granulocytes. It signals through binding and activation of its receptors (CXCR1 and CXCR2).
In addition to its chemotactic and angiogenic properties, it has strong antibacterial activity against Gram-positive and Gram-negative bacteria (approximately 90-fold higher compared to CXCL5 and CXCL7).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.