Thermo Fisher Scientific NR3C1 Monoclonal Antibody (OTI2C4), TrueMAB
NR3C1(Glucocorticoid Receptor) 단백질을 인식하는 마우스 모노클론 항체로, Western blot 및 IHC(P) 검증 완료. 비결합형, 동결건조 형태이며 PBS 버퍼에 보존. 연구용으로만 사용 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2C4 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–265 of human NR3C1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to reach a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Glucocorticoid Receptor (NR3C1) is a receptor for glucocorticoids that functions as a transcription factor and regulator of other transcription factors.
It is typically cytoplasmic until ligand binding induces nuclear transport.
Expressed in various cardiac tissues including atria, ventricles, aorta, and septum.
Alternative splicing and promoter usage generate multiple transcript variants and isoforms.
Mutations in NR3C1 cause glucocorticoid resistance.
GR activation requires association with chaperone proteins and binds steroids such as dexamethasone with nanomolar affinity.
For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.
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