Thermo Fisher Scientific C5AR1 Chimeric Recombinant Rabbit Monoclonal Antibody (32-G1)
C5aR1 인산화형태(Ser334)를 특이적으로 인식하는 재조합 토끼 단일클론 항체. Western blot, ICC/IF, Flow cytometry에 사용 가능. HEK293 발현, Protein A 정제, PBS buffer 보관. 연구용 전용.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG, kappa |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 32-G1 |
| Immunogen | Synthetic C5aR C-terminal phosphopeptide containing phosphoserine at position 334 and neighboring sites Ser-332 and Ser-338 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074732 |
Product Specific Information
Specificity: The 32-G1 antibody recognizes C5aR with phosphorylated serine 334. It specifically reacts with a C5aR peptide containing phosphoserine 334 and with C5aRs isolated from C5a-stimulated, transfected RBL-C5aR cells.
It does not recognize a receptor mutant (C5aR-S--) where all six C-terminal serine phosphorylation sites are replaced with alanine.
Antibody 32-G1 specifically reacts with C5aR phosphoserine 334, independent of neighboring serine residues’ phosphorylation state.
In Bt2cAMP-treated U937 cells, 32-G1 reacts specifically with ligand-activated C5aR at endogenous expression levels but only weakly with unstimulated cells.
Target Information
CD88 (C5aR1) is a C5a Anaphylatoxin Receptor mediating effects of potent chemotactic agents.
C5AR1 is implicated in asthma and expressed in myeloid blood cells, brain, liver, lung, spleen, heart, kidney, and intestinal tract.
CD88 binds the inflammatory peptide anaphylatoxin C5a, stimulating chemotaxis, granule enzyme release, and superoxide production.
It is part of the classical complement cascade and may contribute to tissue injury related to ischemia or sepsis.
Associated diseases include Mannose-Binding Lectin Deficiency and Systemic Lupus Erythematosus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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