Thermo Fisher Scientific C5AR1 Chimeric Recombinant Rabbit Monoclonal Antibody (32-G1)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunocytochemistry (ICC/IF)

10 µg/mL

-

Flow Cytometry (Flow)

1:100

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

32-G1

Immunogen

This antibody was raised by immunizing mice a synthetic C5aR C-terminal phosphopeptide that contained phosphoserine instead of serine at position 334 and also at the neighboring phosphorylation sites Ser-332 and Ser-338. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:C5AR1,uniProtId:P21730-1,ncbiNodeId:9606,antigenRange:334,antigenLength:350,antigenImageFileName:MA5-48290_C5AR1_P21730-1_Rabbit.svg,antigenImageFileNamePDP:MA5-48290_C5AR1_P21730-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3074732

Product Specific Information

Specificity: The 32-G1 antibody recognizes C5aR which has its serine 334 phosphorylated. The mAb 32-G1 reacted specifically with a C5aR peptide that contained phosphoserine 334 and also reacted with C5aRs that were isolated from C5a-stimulated, transfected RBL-C5aR cells. It did not recognize a receptor mutant (C5aR-S--) with alanine replacement of all six C-terminal serine phosphorylation sites. Antibody 32-G1 specifically reacts with C5aR phosphoserine 334, independent of the phosphorylation state of the neighboring serine residues. Studies with Bt2cAMP-treated U937 cells show that 32-G1 specifically reacts with ligand-activated C5aR at endogenous receptor expression levels but only weakly recognizes C5aR isolated from unstimulated cells.

Target Information

CD88, also known as C5aR1, is a C5a Anaphylatoxin Receptor that mediates the effects of one of the most potent chemotactic agents known. C5AR1 has been implicated in asthma, and its expression has been reported in myeloid blood cells, brain, liver, lung, spleen, heart, kidney, and intestinal tract. CD88 is the receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. Functionally, CD88 stimulates chemotaxis, granule enzyme release and superoxide anion production. CD88 is also a component of the classical pathway of the complement cascade; it may be involved with tissue injury associated with ischemia or sepsis and it may be a component of the membrane attack complex. Diseases associated with CD88 include Mannose-Binding Lectin Deficiency and Systemic Lupus Erythematosus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.