Thermo Fisher Scientific CD79b Chimeric Recombinant Rabbit Monoclonal Antibody (HM79-16)
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CD79b 단백질을 인식하는 재조합 토끼 단클론 항체로, WB, IHC, ICC/IF, Flow Cytometry, IP 등에 사용 가능. 마우스 시료 반응성. HEK293 세포에서 발현, Protein A로 정제. 비결합형 액상 형태로 1 mg/mL 농도 제공.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.1 µg/mL |
| Immunohistochemistry (IHC) | Assay-dependent |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| Flow Cytometry (Flow) | 1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG, lambda |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | HM79-16 |
| Immunogen | HM79-16 was initially generated by purifying CD79b from WEHI231 B cells and used for immunisation of footpads of an Armenian hamster. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3074727 |
Product Specific Information
- Specificity: Reacts with an extracellular epitope of CD79b.
- Preparation: HM79-16 was initially generated by purifying CD79b from WEHI231 B cells and used for immunisation of footpads of an Armenian hamster. Hybridoma supernatants were screened for positive reactivity in surface immunofluorescence of WEHI231 and then the specificity to CD79b was tested by immunoprecipitation. Recombinant anti-CD79 antibodies were engineered by cloning and sequencing the full-length IG heavy and light chains of an Armenian hamster (Cricetulus migratorius).
Target Information
CD79b is the B cell antigen receptor Ig beta chain (BCR-Ig beta), which associates with the B cell antigen receptor Ig alpha chain to form a functional heterodimer that interacts with membrane immunoglobulin (mIg). The mIgM component binds antigen and the Ig alpha/Ig beta heterodimer transduces signals.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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