Thermo Fisher Scientific Laminin gamma-1 Monoclonal Antibody (CL3199)
Laminin gamma-1 단백질을 인식하는 Mouse Monoclonal Antibody (CL3199)로, WB, IHC, ICC/IF에 사용 가능. Human 및 Rat 반응성, Protein A 정제, PBS/glycerol buffer에 1 mg/mL 농도로 제공. 연구용으로 basement membrane 관련 연구에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:2,500–1:5,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CL3199 |
| Immunogen | Recombinant protein corresponding to Human Laminin gamma-1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2848917 |
Product Specific Information
Immunogen sequence:
STKAEAERTF AEVTDLDNEV NNMLKQLQEA EKELKRKQDD ADQDMMMAGM ASQAAQEAEI NARKAKNSVT SLLSIINDLL EQLGQLDTVD LNKLNEIEGT LNKAKDEMKV S
Target Information
Laminins are essential and abundant structural non-collagenous glycoproteins localizing to basement membranes. Basement membranes (cell-associated extracellular matrices, ECMs) are polymers of Laminins with stabilizing Type IV Collagen networks, Nidogen, and several proteoglycans. They are found under epithelial layers, around the endothelium of blood vessels, and surrounding muscle, peripheral nerve, and fat cells.
Formation of basement membranes influences cell proliferation, phenotype, migration, gene expression, and tissue architecture. Each Laminin is a heterotrimer of alpha, beta, and gamma chain subunits that undergoes cell secretion and incorporation into the ECM. Laminins can self-assemble, bind to other matrix macromolecules, and have unique and shared cell interactions mediated by Integrins, dystroglycan, and cognate Laminin receptors.
The human Laminin gamma-1 gene maps to chromosome 1q31 and is ubiquitously expressed in tissues that produce basement membranes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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