Thermo Fisher Scientific VIM Monoclonal Antibody (OTI13B1), TrueMAB
Human Vimentin 단백질(aa 210-466)을 면역원으로 제작된 mouse monoclonal antibody. Western blot에 최적화(1:200–1:1,000). Lyophilized 형태로 제공되며, PBS buffer(8% trehalose) 보존. 연구용으로 세포 내 단백질 수송 및 중간 필라멘트 연구에 적합.
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Applications
Western Blot (WB)
- Tested Dilution: 1:200–1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI13B1 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 210–466 of human VIM produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | −20 °C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin.
It plays a role in intracellular protein transport between the nucleus and plasma membrane.
Vimentin, along with desmin, is expressed during early cellular development and later replaced by tissue-specific IFPs.
It contributes to steroid synthesis by serving as a storage network for cholesterol-containing lipid droplets.
Phosphorylation of vimentin by protein kinase leads to filament breakdown and cytoskeletal rearrangement, facilitating cholesterol transport to mitochondria and enhancing steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures or resale without authorization.
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배송/결제/교환/반품 안내
배송 정보
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