Thermo Fisher Scientific Phospho-SKP2 (Thr417) Polyclonal Antibody
Phospho-SKP2 (Thr417) 폴리클로날 항체로 SKP2 단백질의 Thr417 인산화 형태를 특이적으로 검출. Western blot 및 ICC/IF에 사용 가능. 인간, 마우스, 랫트 반응성. 고순도 액상 항체로 안정적 저장 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100–1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human SKP2 (Accession Q13309), corresponding to amino acid residues around phosphorylated Thr417 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817062 |
Product Specific Information
Antibody detects endogenous levels of SKP2/p45 only when phosphorylated at Thr417.
Target Information
Skp2 (S-phase Kinase-associated Protein 2) belongs to the family of F-box proteins that interact with the Cyclin A-Cdk2 complex.
Skp2 is essential for the G1–S transition in both transformed cells and diploid fibroblasts.
It is required for the ubiquitination and degradation of p27 in mammalian cells.
In normal tissues, Skp2 is expressed in tonsil and placenta, while in tumor tissues, it is widely expressed in colon, prostate, pancreas, and skin cancers, with high expression in lung, breast, ovarian, and endometrial cancers.
Research suggests that Skp2 plays a role in oncogenesis, particularly in lymphomas and breast cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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