Thermo Fisher Scientific KCNK16 (TALK-1) (extracellular) Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:600

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

(C)EAWVKGVNPKGNST, corresponding to amino acid residues 75-88 of human KCNK16, 1st extracellular loop if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TALK-1,uniProtId:Q96T55-1,ncbiNodeId:9606,antigenRange:75-88,antigenLength:309,antigenImageFileName:APC-170-200UL_TALK-1_Q96T55-1_Rabbit.svg,antigenImageFileNamePDP:APC-170-200UL_TALK-1_Q96T55-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

0.8 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.4, with 1% BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Information

The KCNK16 gene encodes the TALK-1 channel, an abundant K+ channel transcript found in mouse and in human Beta-cells. There are four human TALK-1 transcript variants, including two that form functional K+ channels (TALK-1a (transcript variant 2) and TALK1-b (transcript variant 3). The TALK-1 structure includes 4 transmembrane domains, 2 extracellular loops, and intracellular N- and C-termini. The C-terminal tail of TALK-1 is a critical component that influences the magnitude of TALK-1 channel activation, while the N-terminus is important for protein interaction. TALK-1, two-pore domain K+ (K2p 16.1) channel is responsible for modulating glucose stimulated insulin secretion that relies on Beta-cell CA2+ influx. The channel produces outwardly rectifying, non-inactivating K+ currents, which are enhanced by elevations in extracellular pH. TALK-1 is thus pH-sensitive, with increased activity under alkaline conditions. In addition, it can be activated by singlet oxygen and nitric oxide.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.