Thermo Fisher Scientific Vitronectin Monoclonal Antibody (HV2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunocytochemistry (ICC/IF)

1:200

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b, kappa

Class

Monoclonal

Type

Antibody

Clone

HV2

Immunogen

Human vitronectin purified from plasma by heparin-affinity chromatography. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Vitronectin,uniProtId:P04004-1,ncbiNodeId:9606,antigenRange:1-478,antigenLength:478,antigenImageFileName:CSI_003-02-02_Vitronectin_P04004-1_House_mouse.svg,antigenImageFileNamePDP:CSI_003-02-02_Vitronectin_P04004-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1088278

Product Specific Information

CSI 003-02-02 is highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin). CSI 003-02-02 is human-specific (no reactivity with cat, dog, cow, sheep, goat, pig, rabbit, horse).

CSI 003-02-02 has been successfully used in ELISA, immunohistochemistry, and Western blotting techniques. CSI 003-02-02 can be used to purify vitronectin from human plasma by affinity chromatography. It can also be used to quantitatively affinity-deplete human plasma or serum of vitronectin. It partially denatures vitronectin upon antibody binding. It binds equally well to native and denatured vitronectin and can be used to quantitate vitronectin in human plasma in a sandwich ELISA with CSI 003-02-02. It does not interfere with the binding of any known vitronectin ligands.

The epitope of CSI 003-02-02 is located in the connecting region. Binding can be competed selectively by a peptide comprising aa 121-133.

NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL

Target Information

VTN is a member of the pexin family. This protein is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. The protein is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.