Thermo Fisher Scientific Vitronectin Monoclonal Antibody (A18)

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent

-

Immunohistochemistry (Frozen) (IHC (F))

Assay-dependent

-

ELISA (ELISA)

1:500

-

Product Specifications

Species Reactivity

Bovine, Horse, Rabbit

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

A18

Immunogen

Lysed bovine corneal endothelial cells and extracellular matrix.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4

Contains

15mM sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1088280

Product Specific Information

CSI 004-18-02 is highly specific for vitronectin. There is no evidence for cross-reactivity with other connective tissue proteins (fibronectin, elastin, collagen, laminin). CSI 004-18-02 cross-reacts with rabbit and horse vitronectin, no reactivity with human or sheep.

CSI 004-18-02 has been successfully used in ELISA and Immunohistochemistry techniques. CSI 004-18-02 is suitable in ELISA and immunostaining of frozen PLP-fixed sections of bovine tissues.

The antibody inhibits integrin-mediated cell adhesion to bovine vitronectin. It can be used to probe vitronectin conformation.

CSI 004-18-02 reacts weakly in ELISA with vitronectin coated directly onto the microtiter plate.

NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL

Target Information

VTN is a member of the pexin family. This protein is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. The protein is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.