Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), Brilliant Violet 711, eBioscience

Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), Brilliant Violet 711, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.25 µg/test

Product Specifications

Additional Formats Available

• Unconjugated
• Alexa Fluor 488, 532, 660, 700
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–805 series
• Brilliant Violet 421, 480, 605, 650, 786
• eFluor 450, 506, 660
• FITC
• Functional Grade
• NovaFluor Blue & Yellow series
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-Cyanine5.5, PerCP-eFluor 710
• Super Bright 436–780 series
• Request custom conjugation

Product Specific Information

The M1/70 monoclonal antibody reacts with mouse CD11b, a 165–170 kDa integrin alphaM.
CD11b forms a non-covalent complex with CD18 (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen.
Expressed by macrophages, NK cells, granulocytes, activated lymphocytes, and B-1 cells in the peritoneal cavity.

• Cross-reactivity: Human CD11b (monocytes, granulocytes, NK subset)
• Reported Application: Flow cytometric analysis
• Tested Application: Flow cytometry on mouse bone marrow cells (≤0.25 µg/test)
• Recommended Optimization: Empirical titration for best assay performance

Brilliant Violet™ 711 (BV711) emits at 713 nm, designed for use with a 405 nm violet laser.
Ensure cytometer compatibility before use.

Buffer Recommendations:

• Use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to reduce polymer dye interactions.

Handling Notes:

• Light-sensitive: protect vial and samples from light.
• Fixation: Compatible with IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C.
• Not compatible with methanol-based fixation.

Excitation/Emission: 407 nm / 713 nm
Laser: Violet Laser

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.

Target Information

CD11b (integrin alpha-M, ITGAM, ITGAX)

• 165 kDa adhesion molecule forming heterodimeric complex with CD18 (Mac-1, CR3).
• Expressed on monocytes/macrophages, granulocytes, activated lymphocytes, NK cells subset, dendritic cells, and microglia.
• Functions as receptor for ICAM-1, ICAM-2, ICAM-4, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X.
• Used as microglial marker in nervous tissue; expression increases during monocyte maturation.
• Associated with systemic lupus erythematosus and ITGAM-related susceptibility.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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