Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), Brilliant Violet 711, eBioscience
Mouse CD11b 검출용 M1/70 클론 단클론 항체로 Brilliant Violet 711 형광체 결합. Flow cytometry에 최적화된 0.25 µg/test 농도로 사용 가능. Mac-1, ICAM-1, C3bi 등과 결합하는 세포 접착 관련 단백질 검출에 적합. Rat IgG2b isotype, 4°C 보관, 빛 차단 필요.
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Thermo Fisher Scientific CD11b Monoclonal Antibody (M1/70), Brilliant Violet 711, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry (Flow)
- Tested Dilution: 0.25 µg/test
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host/Isotype | Rat / IgG2b, kappa |
| Recommended Isotype Control | Rat IgG2b kappa Isotype Control (eB149/10H5), Brilliant Violet™ 711, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | M1/70 |
| Conjugate | Brilliant Violet™ 711 |
| Excitation/Emission Max | 406/711 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2925555 |
Additional Formats Available
- Unconjugated
- Alexa Fluor 488, 532, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395–805 series
- Brilliant Violet 421, 480, 605, 650, 786
- eFluor 450, 506, 660
- FITC
- Functional Grade
- NovaFluor Blue & Yellow series
- PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
- PerCP-Cyanine5.5, PerCP-eFluor 710
- Super Bright 436–780 series
- Request custom conjugation
Product Specific Information
The M1/70 monoclonal antibody reacts with mouse CD11b, a 165–170 kDa integrin alphaM.
CD11b forms a non-covalent complex with CD18 (Mac-1) and binds to CD54 (ICAM-1), C3bi, and fibrinogen.
Expressed by macrophages, NK cells, granulocytes, activated lymphocytes, and B-1 cells in the peritoneal cavity.
- Cross-reactivity: Human CD11b (monocytes, granulocytes, NK subset)
- Reported Application: Flow cytometric analysis
- Tested Application: Flow cytometry on mouse bone marrow cells (≤0.25 µg/test)
- Recommended Optimization: Empirical titration for best assay performance
Brilliant Violet™ 711 (BV711) emits at 713 nm, designed for use with a 405 nm violet laser.
Ensure cytometer compatibility before use.
Buffer Recommendations:
- Use Super Bright Complete Staining Buffer (Product # SB-4401-42) or Brilliant Stain Buffer™ (Product # 00-4409-75) to reduce polymer dye interactions.
Handling Notes:
- Light-sensitive: protect vial and samples from light.
- Fixation: Compatible with IC Fixation Buffer (Product # 00-8222-49) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days at 4°C.
- Not compatible with methanol-based fixation.
Excitation/Emission: 407 nm / 713 nm
Laser: Violet Laser
BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company, used under license. Powered by Sirigen™.
Target Information
CD11b (integrin alpha-M, ITGAM, ITGAX)
- 165 kDa adhesion molecule forming heterodimeric complex with CD18 (Mac-1, CR3).
- Expressed on monocytes/macrophages, granulocytes, activated lymphocytes, NK cells subset, dendritic cells, and microglia.
- Functions as receptor for ICAM-1, ICAM-2, ICAM-4, CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X.
- Used as microglial marker in nervous tissue; expression increases during monocyte maturation.
- Associated with systemic lupus erythematosus and ITGAM-related susceptibility.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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