Thermo Fisher Scientific CD8a Monoclonal Antibody (RPA-T8), Brilliant Violet 711, eBioscience

Applications and Tested Dilution

Product Specifications

Additional Available Formats

Alexa Fluor 532, Alexa Fluor 561, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Brilliant UV 395, Brilliant UV 563, Brilliant UV 615, Brilliant UV 661, Brilliant UV 737, Brilliant UV 805, Brilliant Violet 421, Brilliant Violet 480, Brilliant Violet 605, Brilliant Violet 650, Brilliant Violet 786, eFluor 450, eFluor 506, FITC, NovaFluor Blue 610-70S, NovaFluor Blue 660-120S, NovaFluor Red 710, NovaFluor Yellow 610, NovaFluor Yellow 660, PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610, PerCP-Cyanine5.5, Super Bright 436, Super Bright 600, Super Bright 645, Super Bright 702, Super Bright 780.

Product Specific Information

The RPA-T8 monoclonal antibody reacts with the human CD8a molecule (~32–34 kDa), expressed as a CD8α/β heterodimer or CD8α/α homodimer. CD8a is found on most thymocytes and subsets of mature T and NK cells. It binds MHC class I and associates with tyrosine kinase p56lck, playing a role in T-cell development and activation.

• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-diluted and validated for flow cytometry of human peripheral blood cells (5 µL/test; 0.25 µg/test).
  A test corresponds to staining a 100 µL cell sample (10⁵–10⁸ cells/test).
• Fluorochrome Info: Brilliant Violet™ 711 (BV711) emits at 713 nm; suitable for cytometers with a 405 nm violet laser.
• Buffer Recommendation: Use Super Bright Complete Staining Buffer (#SB-4401-42) or Brilliant Stain Buffer™ (#00-4409-75) when combining polymer dyes to minimize non-specific interactions.
• Light Sensitivity: Protect from light to prevent photo-induced oxidation.
• Fixation: Compatible with IC Fixation Buffer (#00-8222-49) or 1-step Fix/Lyse Solution (#00-5333-54) for up to 3 days at 4°C.
  Not compatible with methanol-based fixation.
• Excitation/Emission: 407 nm / 713 nm (Violet Laser).

BRILLIANT VIOLET™ is a trademark of Becton, Dickinson and Company or its affiliates, used under license. Powered by Sirigen.

Target Information

Cluster of differentiation 8 (CD8) is a type I transmembrane glycoprotein in the immunoglobulin receptor family. It functions as a co-receptor with TCR for MHC class I molecules, mediating signal transduction and T cell activation. CD8 is expressed mainly on cytotoxic T cells, NK cells, monocytes, and dendritic cells. Upon MHC-I/peptide binding, CD8 recruits Lck kinase, leading to cytokine production and cytotoxic T lymphocyte (CTL) activation, crucial for pathogen and tumor clearance. Differentiation of naive CD8+ T cells into CTLs is enhanced by IL-2, IL-12, and TGF-β1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.

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