Thermo Fisher Scientific CD101 Monoclonal Antibody (BB27), Biotin, eBioscience

Thermo Fisher Scientific CD101 Monoclonal Antibody (BB27), Biotin, eBioscience™

Applications

• Flow Cytometry (Flow)
  Tested Dilution: 0.5 µg/test

Product Specifications

Additional Formats

• Unconjugated
• Request custom conjugation

Product Specific Information

The monoclonal antibody BB27 reacts with human CD101, also known as V7 antigen, a member of the immunoglobulin superfamily. CD101 is a disulfide-linked homodimer of unknown function. It is expressed on monocytes, granulocytes, and dendritic cells (Langerhans-like cells expressing HLA-DR, CD1a, CD1c).
Expression on T lymphocytes is associated with cell activation. CD101⁺ CD28⁺ cells respond strongly to CD28 signaling. In combination with anti-CD28 or suboptimal anti-CD3, anti-CD101 can increase proliferation, suggesting an activating role.

The BB27 antibody has been shown to inhibit T cell reactivity in allogeneic and antigen-specific mixed DC-T cell cultures. Mouse CD101 is found on a subpopulation of regulatory T cells (CD4⁺, CD25⁺, Foxp3⁺) with high suppressor activity. In human PBMCs, about 30% of Foxp3⁺ cells express CD101, though enhanced suppressor activity in this population has not been confirmed.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on human peripheral blood cells. Recommended ≤ 0.5 µg per test (100 µL final volume, 10⁵–10⁸ cells/test). Titrate for optimal performance.

Target Information

CD101 (V7 antigen) is a disulfide-linked homodimer expressed on monocytes, granulocytes, dendritic cells, and activated T cells. It plays a potential role in T cell activation and regulation. Mouse CD101 is enriched in regulatory T cells with high suppressor activity, while human CD101 expression correlates with a subset of Foxp3⁺ cells.

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.