Thermo Fisher Scientific Phospho-SHIP1 (Tyr1020) Recombinant Rabbit Monoclonal Antibody (JJ082-03)
Human SHIP1 단백질의 Tyr1020 인산화 부위를 인식하는 재조합 토끼 단클론 항체. WB 및 ICC/IF에 적합하며 높은 특이성과 재현성을 제공. Protein A로 정제된 액상 형태로 4°C 또는 -20°C에서 안정 저장 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JJ082-03 |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Tyr1020 of Human SHIP (aa 991–1040) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2809732 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select the best candidates for production.
Advantages include:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
The SHIP1 gene belongs to the inositol polyphosphate-5-phosphatase (INPP5) family. It encodes a protein with an N-terminal SH2 domain, an inositol phosphatase domain, and two C-terminal interaction domains.
Expression is restricted to hematopoietic cells, where tyrosine phosphorylation mediates movement from the cytosol to the plasma membrane. At the membrane, SHIP1 hydrolyzes the 5′ phosphate from phosphatidylinositol (3,4,5)-trisphosphate and inositol-1,3,4,5-tetrakisphosphate, influencing multiple signaling pathways.
Overall, SHIP1 acts as a negative regulator of myeloid cell proliferation and survival. Alternate splice variants encoding different isoforms have been characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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