Thermo Fisher Scientific KDEL Monoclonal Antibody (2D6), PerCP
KDEL 단백질을 인식하는 마우스 단클론 항체로 PerCP로 표지됨. Western blot에서 1:1,000 희석으로 사용 가능. 인간, 마우스, 랫트 시료에 반응. 단백질 G로 정제된 액상 항체로 4°C에서 보관.
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Applications and Tested Dilution
- Western Blot (WB): 1:1,000
Detects KDEL-tagged proteins in HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2D6 |
| Immunogen | KDEL peptide (C-AVKDEL-COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA) |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932329 |
Additional Formats
Target Information
The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence is located at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins.
GRP78 (78 kDa) and GRP94 (94 kDa) glucose-regulated proteins, as well as protein disulfide isomerase (PDI), all share the C-terminal KDEL sequence.
The presence of a carboxy-terminal KDEL sequence is necessary for ER retention and sufficient to reduce secretion of proteins from the ER. This retention is mediated by a KDEL receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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