Thermo Fisher Scientific KDEL Monoclonal Antibody (2D6), FITC
KDEL 서열을 인식하는 단클론 항체로 FITC 형광 표지되어 있으며, 인간·마우스·랫트 시료에 반응합니다. 단백질 G로 정제된 액상 형태로 제공되며, 4°C 암소 보관이 필요합니다. 웨스턴 블롯에서 1:1,000 희석으로 사용 가능합니다.
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Applications
- Western Blot (WB): Tested dilution 1:1,000
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 2D6 |
| Immunogen | KDEL peptide (C-AVKDEL-COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA) |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 9.1 mM sodium bicarbonate/PBS, pH 7.4, with 640.91 mM DMSO, 136.36 mM ethanolamine |
| Contains | No preservative |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932328 |
Additional Formats
- Unconjugated (MA5-27581)
- APC (MA5-45873)
- PE (MA5-45876)
- PerCP (MA5-45875)
- Custom conjugation available upon request
Product Specific Information
A 1:1,000 dilution of MA5-45874 was sufficient for detection of KDEL in 10 µg of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. Detects KDEL-tagged proteins.
Target Information
The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. GRP78 (78 kDa) and GRP94 (94 kDa), as well as protein disulfide isomerase (PDI), share the C-terminal KDEL sequence. The presence of this sequence is necessary for ER retention and sufficient to reduce secretion of proteins from the ER, mediated by a KDEL receptor.
Usage Notice
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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