Thermo Fisher Scientific CD365 (TIM1) Monoclonal Antibody (1D12-TIM1), PE, eBioscience

Thermo Fisher Scientific CD365 (TIM1) Monoclonal Antibody (1D12-TIM1), PE, eBioscience

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.125 µg)/test

Product Specifications

Additional Formats

• PE-Cyanine7
• Request custom conjugation

Product Specific Information

This 1D12-TIM1 monoclonal antibody recognizes human CD365 (Tim-1, Kim-1, Havcr1). It was generated using the immunoglobulin V domain of the extracellular portion of CD365 and is predicted to recognize all three haplotypes of human CD365.
Clone 1D12-TIM1 has been shown to block HCV virus entry into human hepatoma Huh7.5 cells in a dose-dependent manner and inhibit uptake of apoptotic cells by CD365-expressing 769-P cells.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and tested on A549 cells at 5 µL (0.125 µg) per test.
A test is defined as the amount of antibody that stains a cell sample in 100 µL final volume (10^5–10^8 cells/test).
Excitation: 488–561 nm; Emission: 578 nm; Laser: Blue, Green, Yellow-Green

Target Information

CD365 (T-cell immunoglobulin and mucin domain 1, Tim-1) is a type I surface glycoprotein with an extracellular N-terminal IgV-like domain, a mucin domain, and a short cytoplasmic tail.
Its endogenous ligand is Tim-4. CD365 acts as a receptor or co-receptor for several viruses including hepatitis A/C, Ebola, Marburg, Dengue, Zika, West Nile, and Japanese encephalitis viruses.
Binding occurs via phosphatidylserine on viral envelopes. Polymorphisms in the HAVCR1 gene are linked to infection susceptibility and disease severity.
CD365 is involved in T cell co-stimulation and is primarily expressed in epithelial cells, notably in A549, 769-P, and Caco-2 cell lines.
It also exists as a soluble form generated by proteolytic cleavage.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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