Thermo Fisher Scientific TRF2 Monoclonal Antibody (4A794.15)

Applications

Tested Dilution

Publications

Western Blot (WB)

2-4 µg/mL

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

-

Immunohistochemistry (Frozen) (IHC (F))

5 µg/mL

-

Immunocytochemistry (ICC/IF)

1:10-1:500

-

Flow Cytometry (Flow)

0.1 µg per million cells

View 1 publication 1 publication

ELISA (ELISA)

2 µg/mL

-

Immunoprecipitation (IP)

2 µg per million cells

-

ChIP assay (ChIP)

1:10-1:500

-

in situ PLA (PLA)

Assay-Dependent

-

Product Specifications

Species Reactivity

Deer, Human, Marsupial, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

4A794.15

Immunogen

Full-length TRF2 protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2201326

Product Specific Information

The TRF2 antibody is referred to as both clone 4A794.15 and 4A794 in the published literature.

This antibody recognizes full-length TRF2 as well as TRF2 forms lacking both the N-terminal basic domain (B) and the telobox Myb-like C-terminal DNA-binding domain (M).

TRF2 forms missing the B and M domains are often referred to as mutant TRF2.Although the exact epitope recognized by the TRF2 antibody has not been mapped, the scientific literature indicates it is in the D or L domain, but not in the B or M domain.

Suggested positive control: HL-60, antigen standard for TERF2 (transient overexpression lysate).

Target Information

TRF2 is a ubiquitously expressed protein that is implicated in the control of telomere length. TRF2, like TRF1 contains a Myb-related DNA binding motif. It binds to duplex TTAGGG repeats and is localized to all human telomeres in metaphase chromosomes. TRF2 is thought to protect chromosome ends by maintaining the correct structure at telomere termini. The use of mutant forms of TRF2 has implicated a role for TRF2 in the prevention of senescence in primary human cells. Recently, it has been shown that inhibition of TRF2 resulted in apoptosis in a subset of mammalian cell types.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.