Thermo Fisher Scientific DNMT3A Monoclonal Antibody (64B814.1)
DNMT3A 단백질 검출용 Mouse monoclonal 항체로 Western blot, ICC/IF, Flow Cytometry에 사용 가능. 1 mg/mL 농도의 액상 제품이며, 4°C 또는 -20°C에서 보관. DNA 메틸화 연구 및 ES 세포 발현 분석에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–3 µg/mL |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
| Flow Cytometry (Flow) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 64B814.1 |
| Immunogen | Bacteria expressed HIS-tagged recombinant mouse Dnmt3a |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1074074 |
Product Specific Information
Suggested positive control: antigen standard for DNMT3A (transient overexpression lysate), transfected cell lysate, mouse ES cells.
Target Information
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting, and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. Three families of mammalian DNA methyltransferase genes have been identified: Dnmt1, Dnmt2, and Dnmt3.
Dnmt1 is constitutively expressed in proliferating cells, and its inactivation causes global demethylation and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues, and its inactivation does not affect DNA methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells, downregulated during differentiation, and expressed at low levels in adult somatic tissue.
Mutations in the Dnmt3b gene have been identified in patients with ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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