Thermo Fisher Scientific BCKDK Monoclonal Antibody (OTI11C9), TrueMAB
BCKDK 단백질을 인식하는 Mouse monoclonal antibody (Clone OTI11C9). Western blot, IHC, ICC/IF에 사용 가능. Human 시료 반응성. Lyophilized 형태로 제공되며 재구성 후 약 1 mg/mL 농도로 사용. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI11C9 |
| Immunogen | Full length human recombinant protein of human BCKDK produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The branched-chain alpha-keto acid dehydrogenase complex (BCKD) catalyzes the irreversible second step in the catabolism of branched-chain amino acids (isoleucine, leucine, and valine).
This complex includes three catalytic components:
- E1: branched-chain alpha-keto acid decarboxylase
- E2: dihydrolipoyl transacylase
- E3: dihydrolipoamide dehydrogenase
BCKD activity is regulated by two enzymes: a kinase (BCKDK) and a phosphorylase.
BCKDK contains one histidine kinase domain and is ubiquitously expressed.
Defects in this pathway cause Maple Syrup Urine Disease (MSUD), characterized by accumulation of branched-chain amino acids and their keto acids, leading to encephalopathy and neurodegeneration if untreated.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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