Thermo Fisher Scientific FH Monoclonal Antibody (OTI9G4), TrueMAB
FH 단백질을 표적하는 OTI9G4 클론의 Mouse Monoclonal Antibody. Western blot, IHC, ICC/IF, Flow cytometry 등 다양한 응용에 적합. 고순도 친화 크로마토그래피 정제, 동결건조 형태로 제공. 연구용으로만 사용 가능.
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Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI9G4 |
| Immunogen | Full length human recombinant protein of human FH produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The FH (Fumarase) protein is an enzymatic component of the tricarboxylic acid cycle (Krebs cycle), catalyzing the conversion of fumarate to L-malate. It exists in both cytosolic and mitochondrial forms, differing by translation start site. The mitochondrial form undergoes N-terminal processing to match the cytosolic form. FH functions as a homotetramer and shares similarity with thermostable class II fumarases. Mutations in FH can lead to fumarase deficiency and progressive encephalopathy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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