Thermo Fisher Scientific BrdU Monoclonal Antibody (BU20A), eBioscience
BrdU(5-bromodeoxyuridine) 검출용 Mouse Monoclonal Antibody BU20A. 세포 증식 분석 및 DNA 합성 연구에 적합. Flow cytometry, IHC 등 다양한 응용 가능. 고순도(>90%) 및 낮은 응집도(<10%)로 안정적 성능 제공.
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Thermo Fisher Scientific BrdU Monoclonal Antibody (BU20A), eBioscience
Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-dependent | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 5 publications |
| Flow Cytometry (Flow) | 1 µg/test | View 24 publications |
| In vitro Assay (IV) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Published Species | Chemical |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BU20A |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2865099 |
Additional Formats:
Product Specific Information
This Bu20A monoclonal antibody reacts with 5-bromodeoxyuridine (BrdU), a uridine derivative incorporated into DNA during the S-phase. Anti-BrdU antibodies are used to identify cells that have undergone DNA synthesis during BrdU treatment.
For flow cytometry staining, use the BrdU Staining Buffer Set (Product # 00-5525).
Applications Reported:
- Intracellular staining followed by flow cytometric analysis
- Immunohistology staining of frozen tissue sections
Applications Tested:
- Tested by flow cytometric analysis of BrdU-labeled mouse splenocytes
- Recommended ≤1 µg/test (100 µL final volume, 10⁵–10⁸ cells/test)
Recommended BrdU Labeling and Staining Protocol:
- Label dividing cells with 10 µM BrdU for 45 min at 37°C.
- Wash once with 1X PBS.
- Stain surface molecules as per Surface Staining Protocol.
- Wash in cold Flow Cytometry Staining Buffer or PBS.
- Add 1 mL Foxp3 Fixation/Permeabilization solution (Product # 00-5521-00).
- Incubate 30–60 min at 2–8°C in the dark.
- Wash with 1X Permeabilization Buffer and centrifuge.
- Resuspend in 100 µL Staining Buffer with 30 µg DNase I.
- Incubate 1 hr at 37°C, wash.
- Stain with anti-BrdU antibody 30–60 min, wash.
- If necessary, stain with secondary antibody 30 min, wash.
- Analyze by flow cytometry.
Purity: >90% (SDS-PAGE)
Aggregation: <10% (HPLC)
Filtration: 0.2 µm post-manufacturing filtered
Target Information
Bromodeoxyuridine (BrdU) is a synthetic nucleoside analog of thymidine used to detect proliferating cells. It incorporates into newly synthesized DNA during the S-phase, substituting thymidine. Detection requires DNA denaturation (acid or heat exposure) to allow antibody binding.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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